Recovery of protein kinases from renatured SDS-polyacrylamide gels for biochemical studies.

نویسنده

  • H C Wang
چکیده

1.Connelly, M., C.L. King, K. Bucci, S. Walters, B. Genton, M.P. Alpers, M. Hollingdale and J.W. Kazura. 1997. T-cell immunity to peptide epitopes of liver-stage antigen 1 in an area of Papua New Guinea in which malaria is holoendemic. Infect. Immun. 65:5082-5087. 2.Everest, P., G. Frankel, J. Li, P. Lund, S. Chatfield and G. Dougan. 1995. Expression of LacZ from the htrA, nirB and groE promoters in a Salmonella vaccine strain: influence of growth in mammalian cells. FEMS Microbiol. Lett. 126:97-101. 3.Harris, E.A., A.M. Kenyon, H.D. Nisbet, E.R. Seelye and R.M. Whitlock. 1974. The normal alveolar-arterial oxygen-tension gradient in man. Clin. Sci. Mol. Med. 46:89-104. 4.Hill, A.V.S., J. Elvin, A.C. Willis, M. Aidoo, C.E.M. Allsopp, F.M. Gotch, X.M. Gao, M. Takiguchi et al. 1992. Molecular analysis of the association of HLA-B53 and resistance to severe malaria. Nature 360:434-439. 5.Hormaeche, C.E. and C.M.A. Khan. 1996. Recombinant bacteria as vaccine carriers of heterologous antigens, p. 327-359. In S.H.E. Kaufmann (Ed.), Concepts in Vaccine Development. Walter de Gruyter, Berlin. 6.Kane, J.F. 1995. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr. Opin. Biotechnol. 6:494-500. 7.Khan, C.M.A., B. Villarreal-Ramos, R.J. Pierce, G. Riveau, R. Demarco de Hormaeche, H. McNeill, T. Ali, S. Chatfield et al. 1994. Construction, expression, and immunogenicity of the Schistosoma mansoni P28 glutathione S-transferase as a genetic fusion to tetanus toxin fragment C in a live Aro attenuated vaccine strain of Salmonella. Proc. Natl. Acad. Sci. USA 91:11261-11265. 8.Oxer, M.D., C.M. Bentley, J.G. Doyle, T.C. Peakman, I.G. Charles and A.J. Makoff. 1991. High level heterologous expression in E. coli using the anaerobically-activated nirB promoter. Nucleic Acids Res. 19:2889-2892. 9.Pollack, Y., A.L. Katzen, D.T. Spira and J. Golenser. 1982. The genome of Plasmodium falciparum. I: DNA base composition. Nucleic Acids Res. 10:539-546. 10.Taylor-Robinson, A.W. 1998. Cultivation of Plasmodium falciparum in vitro using microbiological gas sachets that generate microaerophilic conditions. Trans. Roy. Soc. Trop. Med. Hyg. 92:357-358. 11.Zhu, J. and M.R. Hollingdale. 1991. Structure of Plasmodium falciparum liver stage antigen-1. Mol. Biochem. Parasitol. 48:223-226.

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عنوان ژورنال:
  • BioTechniques

دوره 28 2  شماره 

صفحات  -

تاریخ انتشار 2000